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KMID : 1059519870310050457
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Journal of the Korean Chemical Society
1987 Volume.31 No. 5 p.457 ~ p.463
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Synthesis of Insulin A (1-21) Chain and Their Assembly on a Polymer-Bound α-Methylphenacylester Linkage
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Kook Soon-Uoong
Hong Nam-Joo
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Abstract
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The total synthesis of insulin A chain (1-21) with properly protected sulfhdryl groups of three cysteins for the correct intra and inter disulfide bond formation has been accomplished on 2-bromopropionylated 2% DVB-styreneresin support employing manually operated rotary vessel. The sulfhydryl groups of cysteins were protected with acetamidomethyl, benzyl, and benzhydryl respectively. Glutamine and asparagine were attached to the peptide chain by active ester coupling, all other amino acids were coupled with DCC/HOBT. The synthesized peptide was purified by DEAE Sephadex A-25 and gel filtration Sephadex LH-20. The final product was found to be homogeneous by HPLC, electrophoresis, and amino acid analysis. The overall yield of the pure isolated peptide was 6%.
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